A method for linking yeast artificial chromosomes
نویسندگان
چکیده
منابع مشابه
A method for linking yeast artificial chromosomes.
A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the beta-galactosidase gene as an expression reporter in mammalian cells.
متن کاملPCR and yeast artificial chromosomes for sequencing
Laboratoire de GCnCtique Microbienne, lnstitut National de la Recherche Ag ro no m iq ue, Do rn a in e de Vilvert, 78352 Jouy en Josas cedex, France Within the Bacillus subtilis genome sequencing project, the region between /ysA and i/vA was assigned to our laboratory. In this report we present the sequence of the last 36 kb of this region, between the kdg operon and the attachment site of the ...
متن کاملMitotic recombination of yeast artificial chromosomes.
Large regions of human DNA can be cloned and mapped in yeast artificial chromosomes (YACs). Overlapping YAC clones can be used in order to reconstruct genomic segments in vivo by meiotic recombination. This is of importance for reconstruction of a long gene or a gene complex. In this work we have taken advantage of yeast protoplast fusion to generate isosexual diploids followed by mitotic cross...
متن کاملImproved method for the construction of cosmid sublibraries from yeast artificial chromosomes.
Eggleston, S.D. Lauder and W.M. Rehrauer. 1994. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58:401-465. 8.Luckow, B., F. Bunz, B. Stillman, P. Lichter and G. Schutz. 1994. Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans. Mol. Cell. Biol. 14:16261634. 9.Quinn, P.G. and D.K. Granner. 199...
متن کاملA Chlamydomonas genomic library in yeast artificial chromosomes.
We have constructed and characterized a Chlamydomonas reinhardtii total genomic library in yeast artificial chromosomes (YACs). The library contains 7500 clones with inserts ranging in size from 100-200 kb. The representation of the library was assessed by screening one-third of it with a probe derived from the dispersed repeat, Gulliver, which occurs approximately 13 times in the genome. At le...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.21.4192